Summary
Unregisters a folder or database from an ArcGIS Server site.
Syntax
RemoveDataStoreItem (connection_file, datastore_type, connection_name)
| Parameter | Explanation | Data Type | 
connection_file  | For a hosting server, provide the server URL or use the MY_HOSTED_SERVICES keyword. For a stand-alone server, an ArcGIS Server connection file (.ags) representing the server with which you want to register the data.  | String | 
datastore_type  | The type of data being unregistered. 
  | String | 
connection_name  | The name of the folder or database being unregistered, as it is currently registered with the ArcGIS Server site.  | String | 
Code sample
Unregisters a folder from ArcGIS Server that was using the alias "My local data folder".
import arcpy
arcpy.RemoveDataStoreItem("GIS Servers/MyConnection.ags", "FOLDER",
                          "My local data folder")Unregisters a database from ArcGIS Server that was using the alias "MSSQL_Conn".
import arcpy
arcpy.RemoveDataStoreItem("https://webadaptorhost.domain.com/webadaptorname", "DATABASE",
                          "MSSQL_Conn")